Incubation is performed in the microbiological analysis process to promote the growth and proliferation of microorganisms present in a sample. By maintaining the proper temperature and time conditions, microorganisms are allowed to multiply and are easier to detect and identify. This is important in the metal packaging industry, especially in food packaging, to ensure product quality and safety. Incubation helps to verify the presence or absence of undesirable microorganisms that could affect the quality of the packaged product and the health of the consumer.